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Application Notes & Tutorials (9)     Articles & White Papers (6)      Brochures & Specifications (64)      Case Studies (1)      Manuals (28)      Newsletters (4)      Posters (2)      Protocols (77)      References (8)     
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Application Notes & Tutorials (9)

Title Size Pages Hard Copy*
POROS Protein A, G, and CaptureSelect Affinity Columns
Part #: n/a   

POROS® Protein A, Protein G, and CaptureSelect® ligands target multiple IgG epitopes. The graphic shows the binding sites for affinity ligands Protein A and Protein G.
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Optimized blood plasma protocol for profiling miRNA using the OpenArray system
Stock #: CO31609

Real-Time PCR System enables researchers to profile well-characterized and well-studied miRNAs for high-throughput cancer biomarker discovery research, miRNA regulation of mRNA gene expression, and miRNA functional analysis.
435 KB 4  
Single-cell profiling on the OpenArray Real-Time PCR System
Stock #: CO23010

Gene expression profiling using real-time PCR is a pivotal tool for molecular genomics. Typically, RNA or DNA samples originate from tissue composed of heterogeneous cell populations.
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How to Use the Buccal DNA Collector

Remove the Buccal DNA Collector from the pouch, holding the handle at the base. Move Slider Cover back if necessary to fully expose the Collection Paper. Instruct the subject to hold the Buccal DNA Collector and put thumb on the area marked “Thumb” on the back of the Collector.
856 KB 1  
Accurate and Sensitive Mutation Detection and Quantification using TaqMan Mutation Detection Assays
Part #: CO31540   

In this research study, we addressed the feasibility of obtaining sensitive, accurate, and reproducible mutation detection results in synthetic templates, cell lines, and FFPE tissue samples using TaqMan® Mutation Detection Assays.
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Protocol using low sample input for miRNA quantitation on the OpenArray system
Stock #: CO16851

RT primer pools. However, in certain situations where RNA yield is limited, such as with laser capture microdissection or needleaspiration biopsies, allowing reduced RNA input is desirable. We have developed a Low Sample Input (LSI) protocol for these types of reduced-yield samples. To
244 KB 4  
OpenArray digital PCR for virology research - beyond Ct
Stock #: CO31354

However, there are times when qPCR results are ambiguous or inconclusive. Sensitivity or precision may be limited, for example, by extremely low levels of virus. This can cause researchers anxiety and uncertainty over the possibility of virus present but at levels too low to detect.
1887 KB 8  
Attune® Cytometer Applications: Apoptosis
Stock #: CO23104

Annexin V conjugates In apoptotic cells, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment.
1128 KB 5
Protein Thermal Shift Application Note
Stock #: CO31346

The Protein Thermal Shift™ assay measures protein thermal stability using a fluorescent proteinbinding dye. Protein Thermal Shift™ experiments are conducted utilizing the melt capability of Applied Biosystems® real-time PCR systems.
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Articles & White Papers (6)

Title Size Pages Hard Copy*
OpenArray system publications on genotyping and real-time PCR
Stock #: CO31943

Yeung EH, Cuilin Z et al. (2011) Polymorphisms in the neuropeptide Y gene and the risk of obesity: findings from two prospective cohorts. J Clin Endocrinol Metab 96: E2055–E2062. Qi L, Parast L et al.
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Fast real-time PCR is optimized real-time PCR
Stock #: CO13159

Reduced DNA polymerase activation time “Hot-start” thermostable DNA polymerases are strongly recommended for use in real-time PCR. These enzymes are inactive at room temperature and require a heat activation step to become active.
739 KB 4  
GenomeWeb article UCD MiSeq data Validated by Sanger Sequencing

IN ANTICIPATION of a clinical trial involving targeting sequencing of patients with metastatic breast cancer, researchers at the University of California, San Diego, have published some of the first human data using the Illumina MiSeq instrument.
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Life Technologies Submits Sequencer and HLA Typing Kits to FDA for Diagnostic Use-press release

3500 Dx Genetic Analyzer, SeCore® Kits, and uTYPE® Software to raise the standard in sequenced-based HLA typing to identify gene variations commonly used in compatibility selection for transplantation CARLSBAD, Calif.
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Life Technologies and Gen-Probe to Collaborate on Sequencing-Based Assays

Under terms of the agreement, Gen-Probe will commercialize future sequencing-based diagnostic assays and fragment analysis-based human diagnostic genetic tests to be used on the Life Technologies 3500 Dx Series Genetic Analyzers.
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LT Initiates Clinical Trials Leading to 510(k) Submission of a Class I and Class II HLA SBT Solution- Press release

If cleared, Life’s 3500 Dx Genetic Analyzer and SeCore® HLA Sequencing kits would constitute the first 510(k)-cleared sequence-based test for HLA typing in the United States
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Brochures & Specifications (64)

Title Size Pages Hard Copy*
SNaPshot SNP Analysis product bulletin
Stock #: CO110582

Multiplex Kit uses a single-tube reaction to interrogate SNPs at known locations. The chemistry is based on the dideoxy single-base extension of an unlabeled oligonucleotide primer (or primers).
423 KB 4  
Power SYBR® Green PCR Master Mix and Power SYBR® Green RNA-to-CT™ 2-Step Kit
Stock #: CO16930

2-Step Kit deliver highly sensitive DNA, cDNA, and RNA quantitation, detecting as few as 2 copies of a target gene over a broad range of template concentrations. The Power SYBR ® Green PCR Master Mix offers significantly improved sensitivity by employing a highly purified AmpliTaq Gold ®
747 KB 4  
TaqMan® Universal Master Mix II
Stock #: CO16924

Universal Master Mix II brings the quality and reliability you’ve come to expect from Applied Biosystems to a new, improved master mix formulation for real-time PCR.
543 KB 4  
MeltDoctor High Resolution Melt Reagents
Stock #: CO16932

High-resolution melting (HRM) analysis is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves.
1056 KB 4  
Fast SYBR® Green Master Mix
Stock #: CO16936

Green Master Mix delivers highly sensitive and reproducible real-time PCR results in less than half the time when used with Applied Biosystems® fast-enabled real-time PCR instruments. The Fast master mix also offers high specificity by employing the new highly purified AmpliTaq®
2267 KB 4  
Custom TaqMan® Array Cards
Stock #: CO01513

Array Card is a 384-well microfluidic card that enables you to perform 384 simultaneous realtime PCR reactions without the need for liquid-handling robots or multi-channel pipettors to load samples. This highly flexible card enables 1 to 8 samples to be run in parallel against 12 to 384 TaqMan®
1598 KB 6  
POROS Affinity Chromatography Columns Product Bulletin
Part #: CO24355   

Analytical groups supporting biological drug development efforts require rapid, sensitive, and precise methods for quantifying proteins from cell cultures, fermentation harvests, or purification intermediates.
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QuantStudio 12K Flex Product Brochure
Part #: CO31611   

96-well blocks. Block changes typically take less than 1 minute and require no tools, providing the ultimate in format flexibility. Achieve consistent, high-quality results across different plating formats as your research progresses. Scales to your research
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TaqMan PreAmp Master Mix
Stock #: CO110510

To overcome this, methods to preamplify the RNA or cDNA have been developed. These methods, however, are limited in their effectiveness because they inconsistently amplify the starting material, resulting in biased and inaccurate amplification of some transcripts.
405 KB 4  
TaqMan® Copy Number Assays Product Family
Stock #: CO110508

Human genomes vary from one another at the genetic level. Some genetic variations are large, structural, chromosomal variations, while others occur at the singlenucleotide level. A structural variation that has been described in recent years is “copy number variation” (CNV).
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Brochure:Ion PGM and Proton Sequencers
Part #: CO31911   

At the heart of every Ion Torrent™ sequencer is a powerful semiconductor chip. By increasing the density of wells in each generation of chips, Ion Torrent™ sequencers have realized a 100-fold increase in speed and throughput in just their first year. < ION SemIcONductOr SequeNcINg | 01
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TaqMan® Fast Advanced Master Mix
Stock #: CO01509

• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode • Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
586 KB 4
Specification Sheet - Ion PGM Sequencer

Sequencer performs real-time measurements of hydrogen ions produced during DNA replication. Ion semiconductor chips employ a massively parallel array of semiconductor sensors to directly translate genetic information (DNA) to digital information (DNA sequence).
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Ion AmpliSeq Cancer Panel Mutation List (1.0) Spreadsheet

ǂ Ion AmpliSeq Cancer Panel Mutatǂ Geneǂ accession numberǂ COSMIC idǂ cds_mut_syntaxǂ aa_mut_syntaxǂ Strandǂ hg19 coordinatesǂ Insertǂ Target_urlǂ ABL1ǂ X16416ǂ c.730A>Gǂ p.M244Vǂ ǂ 9:133738330-133738330ǂ GGCAGGGGTCTGCACCCGGGAGCCCCCGTTCTATATCATCACTGAGTTCATGACǂ
265 KB 1  
Green Feature Fact Sheet: UltraPure™ Agarose Products
Part #: CO21121   

Life Technologies is committed to designing our products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that these products’ packaging uses less material than their preceding packaging design.
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Green Feature Fact Sheet: Molecular Probes® Pouches
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this products’ packaging uses less material than its preceding packaging design.
163 KB 1  
Green Feature Fact Sheet: MagicMedia™ E. coli Expression Medium SoluPouch™
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product utilizes sustainable packaging principles. The MagicMedia™ E.
161 KB 1  
Green Feature Fact Sheet: GIBCO® Media Bottles
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product utilizes sustainable packaging principles.
167 KB 2  
Green Feature Fact Sheet: Ambion® Cells-to-Ct™ Kits
Part #: CO21121   

™ kits include reagents and enzyme mixtures for reverse transcription (RT) and real-time PCR directly from cultured cell lysates, without the need for a separate RNA isolation step. Green Features
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Green Feature Fact Sheet: SimplyBlue™ SafeStain
Part #: CO21121   

Life Technologies is committed to designing our products with the environment in mind— it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product is less hazardous than comparable products. The use of SimplyBlue™
181 KB 2  
Green Feature Fact Sheet: Click-iT® Detection Assays
Part #: CO21121   

The use of Click-iT® detection assays provides an alternative to exposure to radiation for performing common cellular and biochemical analyses, including detection of cell proliferation and monitoring of protein synthesis and metabolism using the isotopes 3 H-thymidine and 35
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Green Feature Fact Sheet: 7300/7500 Real-Time PCR Systems
Part #: CO21121   

Environmental benefits include reduced use of resources for packaging, thereby improving the packing density and weight, and decreasing greenhouse gas emissions from transport of the same amount of product. Furthermore, less packaging waste is generated at endof-life. Product Description
197 KB 2  
Green Feature Fact Sheet: SimplyBlue™ SafeStain
Part #: CO21121   

Life Technologies is committed to designing our products with the environment in mind— it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product is less hazardous than comparable products. The use of SimplyBlue™
181 KB 2  
Green Feature Fact Sheet: PureLink™ Quick Gel Extraction Kit
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product is now less hazardous than its preceding product.
165 KB 1  
Green Feature Fact Sheet: NovaBright™ Chemiluminescent SEAP Reporter Gene Assay 2.0 Kits
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product is now less hazardous than its preceding product, the NovaBright™
164 KB 1  
Green Feature Fact Sheet: PureLink™ DNA Stabilization Reagent
Part #: CO21121   

DNA Stabilization Reagent can be stored and shipped at a range of temperatures (–80°C to 76°C). This eliminates the need for dedicated freezer space and associated energy use, as well as the use of dry ice and EPS coolers, should there be a need for transport. Product Description
162 KB 1  
Green Feature Fact Sheet: SYBR® Safe DNA Gel Stain
Part #: CO21121   

Life Technologies is committed to designing products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product is less hazardous than the traditional method of DNA staining. SYBR®
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Green Feature Fact Sheet: Click-iT® DIBO Detection and Labeling Reagents
Part #: CO16480   

Life Technologies is committed to designing our products with the environment in mind—it’s one more step toward a smaller footprint. Using Click-iT®
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Application Note: Ion AmpliSeq Cancer Panel
Part #: CO01585   

Targeted resequencing can be utilized for efficient sequencing of specific genes or genomic regions. Current target selection methods are lengthy, complex, and usually require large amounts of input DNA.
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TaqMan® SNP Genotyping Assays
Stock #: CO01515

Assays have the simplest workflow available and are the quickest way to generate genotyping data. Based on powerful TaqMan® probe and primer chemistry and designs, and coupled to dependable Applied Biosystems® instruments and software, these made-to-order assays produce high-confidence results.
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BDD CTRL YIR for detection of mutations in NSCLC

Researchers at the Clinical and Translational Research Core Lab (CTRL), Department of Pathology at the Medical College of Wisconsin in Milwaukee, Wisconsin, are using human tissue samples to perform translational research in order to better understand the underlying mechanisms of disease.
2117 KB 4  
Buccal DNA Collector™ Product Brochure

Human identification laboratories are coping with an increasing volume of reference samples requiring processing for forensic DNA databases, paternity analysis and other applications.
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TargetSeq™ Exome and Custom Enrichment Kit product bulletin
Stock #: CO01309
518 KB 4  
Validation Certificate: AmpFlSTR® Identifiler® Direct PCR Amplification Kit

Direct PCR Amplification Kit for use in Human Identification testing for databasing, and paternity applications. Each laboratory using the AmpFlSTR® Identifiler® Direct PCR Amplification Kit should perform its own appropriate internal validation studies to establish interpretation CO01323
161 KB 1  
Product Bulletin: AmpFlSTR® Identifiler® Direct PCR Amplification Kit

Forensic DNA databases are being created or expanded in many countries worldwide. For example, the National DNA Index System (NDIS) in the United States now contains more than 10 million offender DNA profiles.
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Prep-N-Go Flyer

Buffer is a best-inclass solution designed to enable high quality direct PCR amplification of non-FTA® reference samples without the need for heat incubation, extraction or purification. Prep-n-Go™
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Prep-N-Go Product Insert

Prep-n-Go™ Buffer (Part no. 4467079) is optimized for use with the AmpFlSTR® Identifiler® Direct PCR Amplification Kit (200 reaction kit Part no. 4467831; 1000 reaction kit Part no. 4408580).
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PrepFiler Product Bulletin

The quantity and quality of genomic DNA extracted from a forensic sample can greatly impact the success of the sample analysis process and the overall quality of the final result.
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Validation Certificate: PrepFiler® Automated Forensic DNA Extraction Kit

Life Technologies has validated these kits for use in Human Identification testing for databasing, casework, and paternity applications.
155 KB 1  
Product Flyer: PrepFiler®and PrepFiler® BTA Forensic DNA Extraction Kits

The “BTA” stands for “Bone, Tooth, and Adhesives.” This kit has been developed for the extraction of DNA from calcified tissues (bone, tooth), as well as certain adhesive-containing samples such as cigarette butts, tapelifts and envelope flaps.
1769 KB 4  
Discontinuation Letter High Capacity RNA-to-cDNA Master Mix
Part #: Marketing Material   

Dear valued customer, After careful consideration, Life Technologies has decided to discontinue the manufacture and sale of High Capacity RNA‐to‐cDNA™ Master Mix effective December 31, 2011.
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Specification Sheet: Ion OneTouch System

Instrument are three breakthrough technologies that enable automated delivery of templated Ion Sphere™ particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs.
1120 KB 1  
Green Fact Sheet: AmpliTaq Gold® 360 and AmpliTaq® 360 DNA Polymerases
Part #: CO19451   

In order to minimize the adverse environmental impact of packaging and shipping products on gel ice, Life Technologies investigated the feasibility of shipping its AmpliTaq Gold® 360 and AmpliTaq® 360 DNA Polymerases at ambient temperature.
160 KB 2  
Requires Registration Attune Acoustic Focusing Cytometer Green Feature Fact Sheet
Part #: CO16487   

Life Technologies is committed to designing our products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product produces less hazardous waste than traditional flow cytometry methodology.
152 KB 1  
User Bulletin: Applied Biosystems 3500/3500xL Genetic Analyzer
Part #: 4445098 Rev A   

Related documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Section 1 Troubleshooting issues . . . . . . . . . .
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Requires Registration Attune Acoustic Focusing Cytometer Green Fact Sheet
Part #: CO164870611   

Life Technologies is committed to designing our products with the environment in mind—it’s one more step toward a smaller footprint. This fact sheet provides the rationale behind the environmental claim that this product produces less hazardous waste than traditional flow cytometry methodology.
152 KB 1  
LifeScopeGenomicAnalysisSolutions Spec Sheet
Stock #: CO 01193 Oct 11

Genomic Analysis Solutions build on the accuracy of the 5500 Series Genetic Analyzers with Exact Call Chemistry (ECC), bringing you system-optimized algorithms to generate high-quality data. You can work directly in base space with LifeScope™
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OpenArray® technology on the QuantStudio 12K Flex Real-Time PCR System
Stock #: CO23802

easy-to-use format. For most applications, assays are preloaded onto plates at our state-of-the-art manufacturing facility. To run the plates, simply mix your sample with master mix, load with the automated QuantStudio™ 12K Flex OpenArray® AccuFill™
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QuantStudio 12K Flex REal-Time PCR System with OpenArray Block
Stock #: CO23804

A wide variety of fixed-content and fully customizable plates are available to meet a broad spectrum of research needs. For more information about plate formats, refer to the product bulletin OpenArray® technology on the QuantStudio™ 12K Flex Real-Time PCR System at www.appliedbiosystems.
218 KB 2  
Quick Reference Card: SOLiD™ EZ Bead™ Amplifier
Part #: 4443495 Rev E   

Note: For safety guidelines, refer to the “Safety” section in the Applied Biosystems SOLiD® EZ Bead™ Amplifier Getting Started Guide (PN 4443494). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Application Note: Paired End Sequencing
Part #: CO24084   

Next-generation sequencing — based applications and methodologies are extensively used to investigate genome biology. The lengths of next-generation sequencing reads are limited by accuracy—as read length increases, accuracy decreases.
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PrepSEQ® Residual DNA Sample Preparation Kit Quick Reference Guide
Part #: 4468125 Reva    Stock #: 4468125A

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepSEQ® Residual DNA Sample Preparation Kit Protocol (Part no. 4460663). For every chemical, read the MSDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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resDNASEQ® Vero Residual DNA Data Sheet
Part #: CO23032    Stock #: CO23032

Vero Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero cells (African green monkey), an expression system commonly used for the production of vaccines. Reliable and rapid, the system enables sensitive (LOQ = 1.
925 KB 2  
resDNASEQ® Host Cell DNA Quantitation Systems:CHO, E. coli, Vero, NS0
Part #: CO21719   

Host Cell Residual DNA Quantitation Systems include: • TaqMan® primer and probe mix • TaqMan® master mix • Host cell genomic DNA standard • Negative control Components of the PrepSEQ™ Residual DNA Sample Preparation Kit include: • PrepSEQ™
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TargetSeq™ Custom Enrichment Kit Custom Design Request Form
Part #: 4471869    Stock #: Rev. A

Custom Enrichment Kits. Please complete this form in its entirety to ensure accurate processing of your order. If you have questions, contact !CBD-CSOTeam@lifetech.com. Background information • TargetSeq™
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Target Seq Custom Enrichment Kit Ordering Guide
Part #: 4471870    Stock #: Rev. A

■ Ordering TargetSeq™ Custom Enrichment Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 ■ Submitting custom designs to Life Technologies. . . . . . . . . . . . . . . . . . . . . . . . .
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Red/blue laser flyer: Attune® Acoustic Focusing Cytometer
Stock #: CO31442 0411

Acoustic Focusing Cytometer is the only one of its kind that gives you both precision and sensitivity for your precious samples and rare-event detection. Its no-wash, no-lyse method eliminates cell loss and lets you see results ten times faster than with any other cytometer.
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Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument
Stock #: CO14891

*For in vitro diagnostic use in Australia and New Zealand only. The customer is responsible for any validation of assays, and compliance with any regulatory requirements that pertain to their procedures and instrument use. PRODUCT BROCHURE
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MicroSEQ ID Microbial Identification Software
Part #: CO23108   

Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The new Auto-ID feature in MicroSEQ® ID v3.0 uses a set of editable parameters to automatically identify unknown specimens.
376 KB 2  
Terms and Conditions Diagenode update 2011

Article 1 - Area of Application 1.1. These general conditions are applicable to all of our sales. 1.2 Every purchaser who gives us an order in any way whatsoever will be held to have accepted these general conditions prior to giving the order. 1.
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3500 Dx Series Genetic Analyzer pre-launc Brochure USA
Part #: CO19459   

CO19459 Molokai- Instrument prelaunch brochure.indd 1 7/14/11 4:22 PM The Applied Biosystems® capillary electrophoresis platforms are the industry standard, providing the most reliable, efficient, and widely published technology for DNA sequence analysis.
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Application Note: Targeted Resequencing Guide
Part #: CO19872   

The identification of common and rare variants in candidate regions of the human genome is essential to better understanding the etiology of complex human diseases.
667 KB 4  
BDD UCSF YIR for characterizing melanoma mutations

Melanoma is an aggressive skin cancer with increasing incidence worldwide [1]; it is a malignant tumor arising from pigment-producing melanocytes in the skin, eye, or mucosal surfaces.
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User Bulletin: Applied Biosystems 3500/3500xL Genetic Analyzer

PCR Amplification kit products on the Applied Biosystems 3500 Series Genetic Analyzers, using 3500 Data Collection software Version 1.0 and GeneMapper® ID-X Software Version 1.2 or later.
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Case Studies (1)

Title Size Pages Hard Copy*
Sensitive KRAS Variant Detection by HRM on the Viia 7 Real-Time PCR System
Part #: CO22126   

Somatic mutations of the KRAS gene can lead to a form of the protein that can direct cells to grow and divide without control, implicating KRAS in the development of several types of cancer.
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Manuals (28)

Title Size Pages Hard Copy*
User Guide: RapidMAX Software
Part #: 4469874 RevA   

APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
2742 KB 109  
User Guide: PrepFiler and PrepFiler BTA Forensic DNA Extraction Kits
Part #: 4463348 Rev C   

Publication Part Number 4463348 Rev. C Revision Date January 2012 For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
2903 KB 96  
User Guide: AB Library Builder Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems
Part #: MAN0003622   

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. COPYRIGHT © 2011, Life Technologies Corporation. All rights reserved.
1123 KB 56  
Getting Started Guide: Applied Biosystems ViiA™ 7 Real-Time PCR System
Part #: 4441434 Rev C   

Information in this document is subject to change without notice. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
32935 KB 564  
User Guide: SYBR® Green PCR Master Mix and RT-PCR Reagents Kit
Part #: 4310251 Rev G   

Publication Part Number 4310251 Rev. G Revision Date September 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
661 KB 48  
User Guide: PrepFiler Automated Forensic DNA Extraction Kits
Part #: 4463349 Rev B   

Information in this document is subject to change without notice. LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
7470 KB 144  
User Guide: AmpFlSTR® Identifiler® Direct PCR Amplification Kit
Part #: 4415125 Rev F   

Publication Part Number 4415125 Rev. F Revision Date October 2011 For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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PrepFiler User Bulletin

This user bulletin describes the developmental validation experiments performed to evaluate: • A new wash buffer, PrepFiler™ Wash Buffer B, for use with the PrepFiler™
751 KB 26  
AcroMetrix KRAS FFPE Process Controls Product Insert

KRAS FFPE Process Controls are intended for use in assessing the performance of the isolation, purification, and detection of nucleic acid associated with specific KRAS mutations.
151 KB 2  
Getting Started Guide: SOLiD™ EZ Bead™ Amplifier
Part #: 4443494 Rev E   

Emulsion Cleanup and Bead Enrichment Emulsion PCR Emulsion Preparation For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
2142 KB 47  
User Guide: SOLiD EZ Bead System Software Installation Guide - Software Installation v1.9
Part #: 4472494 Rev A   

Publication Part Number 4472494 Rev. A Revision Date October 2011 For General Laboratory Use. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
1602 KB 15  
Getting Started Guide: SOLiD™ EZ Bead™ Enricher
Part #: 4443496 Rev E   

Emulsion Cleanup and Bead Enrichment Emulsion PCR Emulsion Preparation For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
4458 KB 90  
Getting Started Guide: SOLiD™ EZ Bead™ Emulsifier
Part #: 4441486 Rev E   

Emulsion Cleanup and Bead Enrichment Emulsion PCR Emulsion Preparation For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
3354 KB 81  
Quick Reference Card: SOLiD™ EZ Bead™ Enricher
Part #: 4443497 Rev E   

Note: For safety guidelines, refer to the “Safety” section in the Applied Biosystems SOLiD® EZ Bead™ Enricher Getting Started Guide (Part no. 4443496). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Quick Reference Card: SOLiD™ EZ Bead™ Emulsifier
Part #: 4441487 Rev E   

Note: For safety guidelines, refer to the “Safety” section in the Applied Biosystems SOLiD® EZ Bead™ Emulsifier Getting Started Guide (Part no. 4441486). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
591 KB 6
Applied Biosystems SOLiD EZ Bead System Installation Guide
Part #: 4452500 Rev B   

This user guide is the proprietary material of Life Technologies or its subsidiaries and is protected by laws of copyright. The customer of the SOLiD® EZ Bead™ Enricher, SOLiD® EZ Bead™ Emulsifier, and SOLiD® EZ Bead™
2377 KB 41
NGM SElect Analysis Files for GMIDX Readme

============================================================= About NGM SElect™ Kit Analysis Files v2X for GeneMapper® ID-X Software v1.0.1 and higher Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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NGM SElect Analysis Files for GMID Readme

============================================================= About NGM SElect™ Kit Analysis Files v2 for GeneMapper® ID Software v3.2.1 Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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NGM Analysis Files for GMID Readme

============================================================= About NGM™ Kit Analysis Files v3 for GeneMapper® ID Software v3.2.1 Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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NGM Analysis Files for GMIDX Readme

============================================================= About NGM™ Kit Analysis Files v3X for GeneMapper® ID-X Software v1.0.1 and higher Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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ID Direct Analysis Files for GMIDX Readme

============================================================= About Identifiler® Direct Kit Analysis Files v1 for GeneMapper® ID-X Software v1.0.1 and Higher Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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ID Direct Analysis Files for GMID Readme

============================================================= About Identifiler® Direct Kit Analysis Files v1 for GeneMapper® ID Software v3.2.1 Release Notes Copyright 1999-2011, Life Technologies Corporation. All rights reserved.
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User Guide: Arcturus RiboAmp PLUS RNA Amplification Kit
Part #: 12293-00 Rev B   

APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
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User Guide: Arcturus Paradise PLUS Reagent System
Part #: 12872-00 Rev D   

Information in this document is subject to change without notice. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
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User Guide: AmpFlSTR® NGM™ PCR Amplification Kit
Part #: 4425511 Rev E   

Publication Part Number 4425511 Rev. E Revision Date July 2011 For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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User Guide: Attune™ Acoustic Focusing Cytometer

Publication Part Number 4453328 Rev. E Revision Date August 2011 © Copyright 2011, Life Technologies Corporation. All rights reserved. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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FLUOROPHORE SELECTION GUIDE: Attune® Acoustic Focusing Cytometer

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. © 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Printed in the USA. CO19941 0711
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AmpFlSTR COfiler® PCR Amplification Kit: User Bulletin: Rev F
Part #: 4306116   

NOTICE TO PURCHASER: LIMITED LICENSE The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser.
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Newsletters (4)

Title Size Pages Hard Copy*
POROS Chromatography E-Newsletter Dec 2012
Part #: CO24393   

CaptureSelect® ligands provide alternative specificities and enable rapid binding for the quantitation or purification of biotherapeutic molecules such as IgG, IgM, IgA, Fab, HSA fusions, and IgG Fc fusions. Email bp@lifetech.com to find out which one works for you. For Research Use Only.
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Letter to HID Customers: Approaching Retirement of 3100 Genetic Analyzers (Oct 2011)
Part #: 4336791   

(1) For customers who have an existing AB Assurance contract purchased before September 6, 2011, Technical Support and FAS Support will continue to be available after December 31, 2011. APPROACHING RETIREMENT OF 3100 GENETIC ANALYZERS PLEASE PLAN AHEAD FOR MAXIMAL LAB PRODUCTIVITY
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3100 Retirement Timeline - September 2011
Part #: 4336791   
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August 2011 SOLiD Read Newsletter

Per lane sequencing has arrived Life Technologies proudly announces the arrival of 5500 Series Genetic Analysis Systems with pay-per-lane and application-per-lane sequencing for every researcher. The systems are shipping and being installed at sites now.
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Posters (2)

Title Size Pages Hard Copy*
TargetSeq™ Exome and Custom Enrichment Kit product bulletin
Part #: CO01309   
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Scientific Poster: Expanding the Capabilities of Direct Amplification for Processing Databasing Samples

Laboratories worldwide are developing or expanding forensic DNA databases and are looking for simple, robust, high throughput workflows to process their single source samples. Efficiently.
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Protocols (77)

Title Size Pages Hard Copy*
Quick Reference: OpenArray Sample Tracker Software
Part #: 4460657 Rev B   

■ Product information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 ■ Supported instruments and plates . . . . . . . . . . . . . . . . . . . .
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Quick Reference: RapidMAX Software
Part #: 4469875 RevA   

■ Log in and install assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
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User Bulletin: 5500 Series Genetic Analysis Systems Mate-Paired Library Preparation: 10-kb gDNA inserts
Part #: 4473503 Rev A   

■ Check the size distribution of the library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 2 5500 Series Genetic Analysis Systems Mate-Paired Library Preparation: 10-kb gDNA inserts Preparation of Mate-Paired Libraries Containing 10-kb gDNA Inserts Preface
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Insert: PrepFiler Automated Forensic DNA Extraction Kit
Part #: 4463179 Rev B   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® and PrepFiler® BTA Automated Forensic DNA Extraction Kits User Guide (Part no. 4463349). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Insert: PrepFiler BTA Forensic DNA Extraction Kit
Part #: 4463178 Rev B   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® and PrepFiler® BTA Forensic DNA Extraction Kits User Guide (Part no. 4463348). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Insert: PrepFiler Forensic DNA Extraction Kit
Part #: 4463177 Rev B   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® and PrepFiler® BTA Forensic DNA Extraction Kits User Guide (Part no. 4463348). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Insert: PrepFiler Express BTA Forensic DNA Extraction Kit
Part #: 4442396 Rev C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® Express and PrepFiler® Express BTA Forensic DNA Extraction Kits User Guide (PN 4442699). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Insert: PrepFiler Express Forensic DNA Extraction Kit
Part #: 4442395 Rev C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® Express and PrepFiler® Express BTA Forensic DNA Extraction Kits User Guide (PN 4442699). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Quick Reference: PrepFiler and PrepFiler BTA Forensic DNA Extraction Kits
Part #: 4468126 Rev B   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® and PrepFiler® BTA Forensic DNA Extraction Kits User Guide (Part no. 4463348). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Quick Reference: 5500 Series Genetic Analyzers: Reagents and Consumables Ordering Information
Part #: 4465650 Rev. C   

Publication Part Number 4465650 Rev. C Revision Date December 2011 Use this guide to select the reagents and consumables required to perform a sequencing run on the 5500 Series Genetic Analyzers. • Select Sequencing Primer (SP) kit(s), as required by the library type and desired analysis tag.
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Quick Reference: CopyCaller™ Software
Part #: 4400043 Rev C   

This CopyCaller® Software v2.0 Quick Reference Card contains abridged procedures for using CopyCaller® Software. For the complete procedures, theory of operation, or for safety and biohazard guidelines, refer to the CopyCaller® Software v2.0 User Guide (Part no. 4400042).
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User Guide: CopyCaller™ Software
Part #: 4400042 Rev C   

Real-Time PCR System Applied Biosystems 7900HT Fast Real-Time PCR System Applied Biosystems 7300/7500/7500 Fast Real-Time PCR Systems Applied Biosystems StepOnePlus™ Real-Time PCR System Publication Part Number 4400042 Rev. C Revision Date November 2011 For Research Use Only.
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User Guide: TargetSeq Exome Enrichment System
Part #: MAN0004396 Rev 15 July 2011   

For capturing the exons identified in the VEGA, CCDS, and RefSeq databases and >700 microRNA genes from human genomic DNA for sequencing on the SOLiD™ 4 System or 5500 SOLiD™ Series Systems Catalog Numbers A14060, A14061, A14062, and A14063
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Insert: TargetSeq Exome Enrichment System
Part #: MAN0004567 Rev 7June 2011   

Catalog no. (Probe Pool part no.) Size: A14060 (A14054) 4 reactions A14061 (A14055) 12 reactions A14062 (A14056) 48 reactions A14063 (A14057) 96 reactions Store at –20°C (non-frost-free) Visit www.invitrogen.com/manuals and search for the product catalog number for the complete user guide
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User Bulletin: GeneMapper ID-X Software Version 1.3; Verification Experiments and Installation Procedures
Part #: 4470483 Rev A   

■ Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 About GeneMapper® ID-X Software Version 1.3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Purpose of this user bulletin . . . . . . . . . . . . . . . .
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User Guide: Power SYBR® Green PCR Master Mix and RT-PCR Reagents Kit
Part #: 4367218 Rev E   

Publication Part Number 4367218 Rev. E Revision Date September 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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User Guide: SYBR® Green PCR Core Reagents
Part #: 4304965 Rev F   

Publication Part Number 4304965 Rev. F Revision Date September 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Insert: SYBR Green PCR Master Mix
Part #: 4309158 Rev G   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the SYBR® Green PCR Master Mix and SYBR® Green RT-PCR Reagents Kit User Guide (PN 4310251). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Insert: Power SYBR Green PCR Master Mix
Part #: 4367656 Rev C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Power SYBR® Green PCR Master Mix and Power SYBR® Green RTPCR Reagents Kit User Guide (PN 4367218). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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Single Cell Lysis Kit Product Insert
Part #: 4458355 Rev D   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Single Cell-to-CT™ Kit User Guide (Part no. 4458356). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Single Cell-to-CT™ Kit Product Insert
Part #: 4458354 Rev D   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Single Cell-to-CT™ Kit User Guide (Part no. 4458356). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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PrepSEQ™ Sample Preparation Kits
Part #: 4465875 Rev. A   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepSEQ® Sample Preparation Kits User Guide (Part No. 4465957). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Protocol for Mycoplasma Nucleic Acid Extraction Kits
Part #: 4465957 Rev. A   

Publication Part Number 4465957 Rev. A Revision Date August 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Quick Reference Card for MycoSEQ™ Mycoplasma Detection Kits
Part #: 4465876 Rev. A   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the MycoSEQ™ Mycoplasma Detection Kits User Guide (PN 4465874). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. ■ Procedure . . . . .
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Protocol for MycoSEQ™ Mycoplasma Detection Kits
Part #: 4465874 Rev. A   

Publication Part Number 4465874 Rev. A Revision Date August 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Bovine Virus Diarrhea RNA Test Kit Instructions
Part #: 4425603 Rev C   

The Bovine Virus Diarrhea RNA Test Kit (VetMAX™-Gold BVDV Detection Kit, PN 4413938) is intended for use in the rapid, in vitro detection of bovine virus diarrhea virus (BVDV) RNA extracted from ear punch samples obtained from acutely and persistently infected (PI) cattle.
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Quick Reference: PrepFiler® Automated Forensic DNA Extraction Kit
Part #: 4468127 Rev A   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the PrepFiler® Automated Forensic DNA Extraction Kit User Guide (Part no. 4463349). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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resDNASEQ® Quantitative DNA Kits User Guide
Part #: 4460662 Rev. B    Stock #: 4460662B

Publication Part Number 4460662 Rev. B Revision Date May 2011 For research use only. Not intended for any human or animal therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Quick Reference: Ion Total RNA-Seq Kit for Small RNA Libraries
Part #: 4467092 Rev C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Ion Total RNA-Seq Kit User Guide (PN 4467098). For every chemical, read the Safety Data Sheet (SDS) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Quick Reference: Ion Total RNA-Seq Kit for Whole Transcriptome
Part #: 4467091 Rev C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Ion Total RNA-Seq Kit User Guide (PN 4467098). For every chemical, read the Safety Data Sheet (SDS) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
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Protocol: Ion Total RNA-Seq Kit
Part #: 4467098 RevC   

Publication Part Number 4467098 Rev. C Revision Date October 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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resDNASEQ® Quantitative DNA Kits Quick Reference Guide
Part #: 4468124 RevA    Stock #: 4468124A

Note: For safety and biohazard guidelines, refer to the “Safety” section in the resDNASEQ® Quantitative DNA Kits User Guide (Part no. 4460662). For every chemical, read the SDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. Product overview
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PrepSEQ® Residual DNA Sample Preparation User Guide
Part #: 4460663Rev. B    Stock #: 4460663B

Publication Part Number 4460663 Rev. B Revision Date May 2011 For research use only. Not intended for any human or animal therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Requires Registration Protocol: TaqMan Salmonella enterica Kit Protocol
Part #: 4381939 Rev D   

Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document.
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Product Insert: BigDye Terminator v1.1 Matrix Standard (Dye Set E)
Part #: 4363110 Rev C   

©Copyright 2010. Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Product Insert October 2010
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Silencer® Select, Ambion In Vivo, Custom Select siRNA, In Vivo Ready - Product Insert
Part #: 4457172 Rev C   

Silencer® Select Pre-designed and Validated siRNA, In Vivo Ready Ambion® In Vivo Pre-designed and Custom Designed siRNA, In Vivo Ready or HPLC-purified Custom Select siRNA and Ambion® In Vivo Custom siRNA, In Vivo Ready
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Silencer® Select Pre-designed, Validated, and Custom Designed siRNA - Insert
Part #: 4457171 Rev B   

Silencer® Select Pre-designed, Validated, and Custom Designed siRNA Custom Select siRNA Ambion® In Vivo Pre-designed, Custom Designed, and Custom siRNA, Standard Purity Note: For all reagents, read the Safety Data Sheet (SDS) and follow the handling instructions.
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Protocol: TaqMan® Fast Universal PCR Master Mix (2X)
Part #: 4351891 Rev G   

Publication Part Number 4351891 Rev. G Revision Date August 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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Product Insert: TaqMan® Fast Universal PCR Master Mix (2X), No AmpErase UNG
Part #: 4357649 Rev D   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the TaqMan® Fast Universal PCR Master Mix (2✕), No AmpErase® UNG (Part no. 4351891). For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions.
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SOLiD™ Total RNA-Seq Kit Protocol
Part #: 4452437 Rev B   

Publication Part Number 4452437 Rev. B Revision Date July 2011 For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
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siPORT™ NeoFX™ Transfection Agent
Part #: 4510M Rev D   

Product description siPORT™ NeoFX™ Transfection Agent is a lipid-based formulation that provides extremely high levels of transfection without inducing cellular stress that can lead to cytotoxicity. It can be used with a wide range of cell types† and under diverse growth conditions.
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Product Insert: VetMAX™ M. hyopneumoniae Controls
Part #: 4425906 Rev C   

The VetMAX™ M. hyopneumoniae Controls is a mixture of Mycoplasma hyopneumoniae control DNA (1000 copies/µL) and Xeno™ DNA Control (1000 copies/µL) that serves as a positive control for amplification of M. hyopneumoniae DNA and Xeno DNA Control in multiplex real-time PCR.
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Product Insert: VetMAX™ M. hyopneumoniae Reagents - Applied Biosystems Design
Part #: 4425905 Rev C   

The VetMAX™ M. hyopneumoniae Reagents is a proprietary primer and probe mix designed to amplify Mycoplasma hyopneumoniae DNA and Xeno™ DNA Control in multiplex realtime PCR. Use 1 µL of the VetMAX M. hyopneumoniae Reagents per 25-µL reaction. TaqMan® Probe Reporter/Quencher
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Product Insert: VetMAX M. hyopneumoniae Reagents
Part #: 4425903 Rev C   

The VetMAX™ M. hyopneumoniae Reagents is a primer and probe mix designed to amplify Mycoplasma hyopneumoniae DNA and Xeno™ DNA Control in multiplex real-time PCR. The primer and probe designs for M. hyopneumoniae DNA amplification are published and publicly available. Use 1 µL of the VetMAX M.
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Product Insert: VetMAX T. foetus Controls
Part #: 4425902 Rev C   

The VetMAX™ T. foetus Controls is a mixture of Tritrichomonas foetus control DNA (1000 copies/µL) and Xeno™ DNA Control (1000 copies/µL) that serves as a positive control for amplification of T. foetus DNA and Xeno DNA Control in multiplex real-time PCR. Use 8 µL of the VetMAX T.
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Insert: VetMAX T. foetus Reagents
Part #: 4425901 Rev C   

The VetMAX™ T. foetus Reagents is a primer and probe mix designed to amplify Tritrichomonas foetus DNA and Xeno™ DNA Control in multiplex real-time PCR. The primer and probe designs for T. foetus DNA amplification are published and publicly available. Use 1 µL of the VetMAX T.
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VetMAX BTV Reagents PI Sheet
Part #: 4425898 Rev C   

The VetMAX™ Bluetongue Virus Reagents is a primer and probe mix designed to amplify bluetongue virus (BTV) RNA and Xeno™ RNA Control in multiplex real-time RT-PCR. The primer and probe designs for BTV RNA amplification are published and publicly available.
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VetMAX BRSV Controls PI Sheet
Part #: 4425884 Rev C   

The VetMAX™ BRSV Controls is a mixture of bovine respiratory syncytial virus (BRSV) control RNA (1000 copies/µL) and Xeno™ RNA Control (1000 copies/µL) that serves as a positive control for amplification of BRSV RNA and Xeno RNA Control in multiplex real-time RT-PCR.
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VetMAX BRSV Reagents PI Sheet
Part #: 4425879 Rev C   

The VetMAX™ BRSV Reagents is a primer and probe mix designed to amplify bovine respiratory syncytial virus (BRSV) RNA and Xeno™ RNA Control in multiplex real-time RT-PCR. The primer and probe designs for BRSV RNA amplification are published and publicly available.
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VetMAX IBR Controls PI Sheet
Part #: 4425882 Rev C   

The VetMAX™ IBR/BHV-1 Controls is a mixture of bovine herpesvirus 1 (BHV-1) control DNA (1000 copies/µL) and Xeno™ DNA Control (1000 copies/µL) that serves as a positive control for amplification of BHV-1 DNA and Xeno DNA Control in multiplex real-time PCR.
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VetMAX IBR Reagents PI Sheet
Part #: 4425817C   

The VetMAX™ IBR/BHV-1 Reagents is a primer and probe mix designed to amplify bovine herpesvirus 1 (BHV-1) DNA and Xeno™ DNA Control in multiplex real-time PCR. The primer and probe designs for BHV-1 DNA amplification are published and publicly available.
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Protocol: TaqMan® Fast Virus 1-Step Master Mix
Part #: 4453800 Rev B   

Information in this document is subject to change without notice. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
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User Bulletin: ViiA 7 Real-Time PCR System
Part #: 4467664 Rev A   

Overview New 96-well block users currently using ViiA™ 7 Software v1.0 are required to upgrade to ViiA™ 7 Software v1.1 in order to calibrate their new 96-well block. In this user bulletin This user bulletin covers: ■ Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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SOLiD 5500 Protocol for Tecan EVO75 Updated

Freedom EVO and Freedom EVOware are registered trademark of Tecan Group Ltd. AMPure is a registered trademark of Beckman Coulter, Inc. Covaris is a registered trademark of Covaris, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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SOLiD4 Protocol for Tecan EVO75 Updated

Freedom EVO and Freedom EVOware are registered trademark of Tecan Group Ltd. AMPure is a registered trademark of Beckman Coulter, Inc. Covaris is a registered trademark of Covaris, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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SOLiD4 Exd file for Tecan EVO150

Transfer_Rack("28","22",1,0,1,0,0,"","Eppendorf 96/500 DNA LoBind","Narrow","","","AB MP 3Pos","","Te-Shake 1Pos 2 MP SOLiD","2","(Not defined)","2"); WaitTimer("4","420"); GroupEnd(); Group("Size Selection 2"); Variable(SAMPLE_CNT,"SAMPLE_CNT",0,"",0,1.000000,24.000000,0,2,0,0);
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SOLiD4 Adaptor Calculator for Tecan EVO150

Tecan EVO Applied Biosystems® SOLiD™ 4 System Adaptor Calculator Tool Instructions: Fill in library information in white cells. Calculated values will automatically appear in yellow cells. Platform: SOLiD™ 4 Standard Standard Final PCR Reaction Volume Protocol: Express Barcoded Sample
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SOLiD4 Frag Protocol for Tecan EVO150

Freedom EVO and Freedom EVOware are registered trademark of Tecan Group Ltd. AMPure is a registered trademark of Beckman Coulter, Inc. Covaris is a registered trademark of Covaris, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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SOLiD 5500 Frag EXD for Tecan Evo150

Transfer_Rack("28","22",1,0,1,0,0,"","Eppendorf 96/500 DNA LoBind","Narrow","","","AB MP 3Pos","","Te-Shake 1Pos 2 MP SOLiD","2","(Not defined)","2"); WaitTimer("4","420"); GroupEnd(); Group("Size Selection 2"); Variable(SAMPLE_CNT,"SAMPLE_CNT",0,"",0,1.000000,24.000000,0,2,0,0);
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SOLiD 5500 Frag for Tecan EVO 150

Freedom EVO and Freedom EVOware are registered trademark of Tecan Group Ltd. AMPure is a registered trademark of Beckman Coulter, Inc. Covaris is a registered trademark of Covaris, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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Product Insert: AmpFlSTR® Identifiler® Direct PCR Amplification Kit (200 reactions)
Part #: 4468050 Rev A   

IMPORTANT! Reagents used for amplification must be isolated from any source of contaminating DNA, especially from previously amplified AmpFlSTR® PCR products and the AmpFlSTR® Allelic Ladders. Thaw reagents completely before first use.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Swedish Translation
Part #: 4461395 Rev B   

För riktlinjer om säkerhet och biologiska risker, se avsnittet "Safety" i TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429). För varningar och försiktighetsåtgärder, se bipacksedeln TrueScience™ RespiFinder® Identification Panels Product Insert (PN 4460486).
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Turkish Translation
Part #: 4461394 Rev B   

Güvenlik ve biyolojik tehlike yönergeleri için TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429) “Safety” kısmına bakın. Uyarılar ve önlemler için, TrueScience™ RespiFinder® Identification Panels Product Insert'e (PN 4460486) başvurun.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Spanish Translation
Part #: 4461393 Rev B   

Si desea conocer las recomendaciones de seguridad y riesgos biológicos, consulte el apartado “Safety” en el documento TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429).
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Russian Translation
Part #: 4461379 Rev B   

Указания по безопасности и биологической опасности приведены в разделе «Safety» в TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429).
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Portuguese Translation
Part #: 4461378 Rev B   

Identification Panels Product Insert (PN 4460486). Para cada produto químico, leia as fichas de dados de segurança (SDS) e siga as instruções de manipulação. Use óculos, vestuário e luvas de protecção adequados.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Polish Translation
Part #: 4461377 Rev B   

RespiFinder® Identification Panels Protocol (PN 4460429). Informacje dotyczące ostrzeżeń i środków ostrożności można znaleźć w dokumencie TrueScience™ RespiFinder® Identification Panels Product Insert (PN 4460486).
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Korean Translation
Part #: 4461376 Rev B   

안전 및 생물학적 유해 물질 관련 수칙은 TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429) 의 "Safety" 섹션 을 참조하십시오 .
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Japanese Translation
Part #: 4461375 Rev B   

appliedbiosystems.com/respifinderlit をご参照ください。 TrueScience™ RespiFinder® Identification Panels ユーザーガイドを ダウンロードするには、www.appliedbiosystems.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Greek Translation
Part #: 4461374 Rev B   

Για κάθε χημική ουσία, διαβάστε το φύλλο δεδομένων ασφαλείας (SDS) και ακολουθήστε τις διαδικασίες χειρισμού.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - German Translation
Part #: 4461373 Rev B   

Die Richtlinien zur Sicherheit und Biogefährdung finden Sie im Abschnitt „Safety“ im TrueScience™ RespiFinder® Identification Panels Protocol (PN 4460429). Warnungen und Sicherheitsmaßnahmen finden Sie im TrueScience™ RespiFinder® Identification Panels Product Insert (PN 4460486).
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - French Translation
Part #: 4461372 Rev B   

Identification Panels Product Insert (PN 4460486). Concernant tous les produits chimiques, lire la fiche signalétique et suivre les consignes de manipulation. Porter des protections oculaires, des gants et des vêtements appropriés.
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TrueScience™ RespiFinder® Identification Panels Quick Reference Card - Arabic Translation
Part #: 4461370 Rev B   

TrueScience™ RespiFinder® Identification Panels Headquarters 5791 Van Allen Way | Carlsbad, CA 92008 USA Phone 760.603.7200 www.lifetechnologies.com Translated from the English PN 4460430 Rev. A Part Number 4461370 Rev. B 07/2011
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Quick Reference Card: Applied Biosystems® TrueScience™ Aneuploidy STR Kits
Part #: 4457193C   

Note: For safety and biohazard guidelines, refer to the “Safety” section in the Applied Biosystems® TrueScience™ Aneuploidy STR Kits Protocol (PN 4454039). For warnings and precautions, refer to the Applied Biosystems® TrueScience™ Aneuploidy STR Kits Product Insert (PN 4453329).
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Protocol: Applied Biosystems® TrueScience™ Aneuploidy STR Kits
Part #: 4454039 Rev C   

Publication Part Number 4454039 Rev. C Revision Date July 2011 The results obtained from these or any other diagnostic kits should be used and interpreted only in the context of the overall clinical picture. Life Technologies cannot accept responsibility for any clinical decisions that are made.
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Product Insert: Applied Biosystems® TrueScience™ Aneuploidy STR Kits
Part #: 4453329 Rev D   

To obtain documents, go to www.appliedbiosystems.com/ aneuploidy, then click the link for [Support], or contact your local Life Technologies representative. For safety and biohazard guidelines, refer to the “Safety” section in the TrueScience™ Aneuploidy STR Kits Protocol (PN 4454039).
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References (8)

Title Size Pages Hard Copy*
Assay Index File v18 for miRNA (excel)

bfl-miR-96::bta-miR-96::cfa-miR-96::dre-miR-96::eca-miR-96::fru-miR-96::hsa-miR-96-5p::mdo-miR-96::mmu-miR-96-5p::oan-miR-96::ppy-miR-96::rno-miR-96::tni-miR-96::xtr-miR-96
3685 KB 1  
Assay Index File v18 for miRNA (txt)

2)::rno-miR-290(15) CUCAAACUAUGGGGGCACUUUUU INV 000189 mmu-miR-295 mmu-miR-295-3p MIMAT0000373 mmu-miR-295(17) AAAGUGCUACUACUUUUGAGUCU INV 000190 mmu-miR-298 mmu-miR-298(9.2)::rno-miR-298(9.
1516 KB 1  
Custom TaqMan® Array Card Formats: Miscellaneous
Stock #: 127MI57-03

Format 12 (Part no. 4342247) 11 unique assays 1 mandatory control 8 unique samples Format 24 (Part no. 4342249) 23 unique assays 1 mandatory control 8 unique samples Format 16 (Part no. 4346798) 15 unique assays 1 mandatory control 8 unique samples Format 32 (Part no. 4346799) 31 unique
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GeneMapper® ID-X Software Hotfix_20110620 Release Notes

ID-X Software Hotfix_20110620 (“Hotfix_20110620”) * System Requirements * Before Installation of Hotfix_20110620; Special Considerations * Installation of Hotfix_20110620 for GeneMapper® ID-X Software v1.0.1 * Installation of Hotfix_20110620 for GeneMapper® ID-X Software v1.
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AmpFlSTR® NGM™ PCR Amplification Kit - Overview

− Improved buffer formulation overcomes significant levels of inhibition and supports enhanced profile and locus balance, even when inhibitors are present − Concentration of loci in the low molecular weight region of the profile increases the power of discrimination in partial profiles
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Product Information Sheet: Attune™ performance tracking beads

Format,1,2,,,,,,,,,,,,,, Culture,en-US,,,,,,,,,,,,,,, Lot Number,756080,,,,,,,,,,,,,,, Product Name,4 Peak Beads,,,,,,,,,,,,,,, Expiration Date,5/20/2021,,,,,,,,,,,,,,, FSC bead ratio,0.
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Centrifuge Compatibility Chart
Stock #: CO11312

Centrifuge compatibility Previous-generation centrifuges Current vendor Centrifuge Centrifuge rotor Applied Biosystems bucket for TaqMan® Array Sorvall Legend T Plus, 120 V Thermo-Fisher 75004367 75006445 (includes required tool) or 75006434 (twist-on) 4337762 Sorvall Legend T Plus, 230 V
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SOLiD 5500 Adaptor Calculator for Tecan Evo150

Fill in library information in white cells. Calculated values will automatically appear in yellow cells. A 1:10 dilution is required for values in red. Platform: SOLiD™ 5500 Standard Standard Final PCR Reaction Volume Protocol: Express Barcoded Sample DNA Position
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