Small non-coding RNAs are typically only ~18–40 nucleotides in length, however their effect on cellular processes is profound (see Figure 1). Small RNA has been shown to play critical roles in developmental timing, cell fate, tumor progression, neurogenesis.

Animals, plants, and fungi contain several distinct classes of small RNA, including:

• microRNA (miRNA)
• short interfering RNA (siRNA)
• piwi-interacting RNA (piRNA)
• siRNA RNA (rasiRNA)

Small RNA Analysis with the SOLiD™ System
The SOLID Small RNA Solution is a robust method for hypothesis-neutral, whole genome analysis of expression patterns that enables you to discover novel RNA without the probe bias of microarrays.
 
Create a small RNA library in a single day.
Using the SOLiD™ Small RNA Expression Kit, you can  convert total RNA into a library suitable for emulsion PCR in a single day using 5 easy steps and a single purification (Figure 2). This simplified protocol reduces experimental variability and improves the detection of biologically relevant changes.
 
Detect allele specific expression patterns.
This novel approach to library creation enables you to conserve the strand orientation of the original RNA molecule.  For the first time you will know exactly which strand a particular transcript originated from.

Detect as little as 1 copy of small RNA per cell.
The SOLiD™ 3 System’s ultra-high throughput (greater than 600 million mappable sequence reads, or tags, per run), wide dynamic range, and high sensitivity make it particularly suited for analyzing low RNA expression levels and measuring accurate fold changes at the same low expression levels.

Learn More
Learn about the workflow for Whole Genome Analysis of Small RNA (PDF, 1.5 MB).