AB SCIEX MALDI Imaging Solutions for Peptides and Proteins

MALDI mass spectrometry imaging (MSI) is an emerging technology that is being used to visualize the spatial distribution of small molecules, peptides and proteins directly from the surface of a tissue section. Unlike traditional autoradiography or immunochemical imaging, MSI can analyze multiple analytes without the need for labeling, and identification can be obtained using MS/MS.

The TOF/TOF MALDI imaging Solution consists of sample preparation, the market leading 4800 Plus MALDI TOF/TOF™ System, and TissueView™ software for advanced data processing and visualization.

The AB SCIEX TOF/TOF™ 5800 system has the unique on-axis 1000 Hz Nd:YAG OptiBeam™ laser for unsurpassed sensitivity to detect low abundant analytes – from small molecules to peptides and proteins.

The superior QuanTIS™ selection of precursor-ions prior to fragmentation provides clean, database-searchable MS/MS spectra for the identification of peptides and characterization of metabolites.

MSI Intensity Maps from Coronal Section of Rat Brain

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• Spatial resolution: Variable from 500 µm down to 1 µm with over-sampling
• Speed: Acquire spectra with 200 Hz laser frequency
• MS/MS precursor selection: ± 1.25 Da at 1,000 m/z without any loss in sensitivity
• Unique heated source minimizes contamination and reduces downtime