Top Tutorials, Guides & Troubleshooting Documents

DNA Sequencing and Fragment Analysis

Getting Started Guides


• Automated DNA Sequencing Chemistry Guide (3.06MB PDF)
• Genescan® Reference Guide: Chemistry Reference for the ABI Prism® 310 Genetic Analyzer (1.9MB PDF)



Training


• DNA Sequencing Training
• Fragment Analysis Training
• Training Calender



Fragment Analysis/Genotyping Applications


• Application Note: AFLP Analysis on Applied Biosystems Capillary Electrophoresis Systems ( 229KB PDF)
• Application Note: BAC Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer ( 3.22MB, PDF)
• Fact Sheet: Microsatellite Analysis on the 3130 Series Systems ( 491KB, PDF)
• Application Note: Quanititative Multiplex PCR-based Assay: Detection of Large Deletions or Duplications in Genomic DNA Using Quantitative Multiplex PCR-Based Assay on Applied Biosystems Capillary Electrophoresis Systems (5.07MB, PDF)
• Application Note: Relative Fluorescent Quantitation on the 3130 Series Systems (907KB, PDF)
• SNaPShot™ Multiplex Kit
• SNPlex
• Application Note: Single-Stranded Conformation Polymorphism (SSCP) on Applied Biosystems Capillary Electrophoresis Systems (319KB, PDF)
• Application Note: Terminal Fragment Length Polymorphism (T-RFLP) on Applied Biosystems Capillary Electrophoresis Systems (216KB, PDF)



DNA Sequencing Applications


• Application Note: A Workflow for Obtaining High Quality Sequencing Data from Bacterial Artificial Chromosome (BAC) DNA ( 631KB, PDF)
• Application Note: MLST: A Fast, Accurate, and Automated Workflow for Multi Locus Sequence Typing of Bacterial Isolates ( 551KB, PDF)
• MitoSEQr™ Resequencing System Protocol Summary (KB, PDF)
• VariantSEQr™ Resequencing System: Protocol Summary (NCBI Search for Primers) ( 1.54MB, PDF)



Instrument Guides



310 Genetic Analyzer


• Autosampler Calibration (635k PDF)
• Resolving Communication Problems (1 MB PDF)
• Test CCD 4 Color and Seq Fill Modules (840k PDF)
• View more documents >



3100-Avant and 3100 Genetic Analyzer


• 3130/3130xl Genetic Analyzers Maintenance, Troubleshoooting, and Reference Guide, Rev. D (3.37MB, PDF)
• 3130/3130xl Genetic Analyzers Getting Started Guide, Rev. C (10.54MB, PDF)
• User Bulletin: Introducing New 96-Well Fast Plate Adapters for Applied Biosystems Capillary Electrophoresis Systems, Rev. C (1.14MB, PDF)



3130 and 3130xl Genetic Analyzer

3730 and 3730xl DNA Analyzer


Analysis Software Guides



GeneMapper® Software v4.0


• GeneMapper® Software v.4.0 with Multi-User Client-Server Capability (437KB, PDF)



Sequencing Analysis Software


• Sequencing Analysis Software v.5.3 User Bulletin, Rev. C (382KB, PDF)
• Frequently Asked Questions: KB™ Basecaller v.1.4 (166KB) PDF)



SeqScape® Software


• Applied Biosystems SeqScape® Software v2.5: Quick Reference Guide: Rev A (333k PDF)
• SeqScape® Software Version 2.5: Frequently Asked Questions: Rev A (334k PDF)



Troubleshooting


• Troubleshooting Computer Systems: Windows® 2000 and Windows® XP Computer Systems: Technical Support Bulletin 1 (199 KB PDF)
• Technical Support Bulletin 2: Submitting and Receiving Data to and from Applied Biosystems Genetic Analysis Technical Support, Rev. A (568KB, PDF)
  
  

PCR

Applications and Workflow




• PCR Optimization: Reaction Conditions and Components (123.8 KB PDF)
• Quantitative Analysis of the PCR Reaction (81.6 KB PDF)



Thermal Cycler and Software Guides



GeneAmp® PCR System 2700 and 2720

GeneAmp® PCR System 9700

Veriti™ Thermal Cycler

Protein Quantification

Cleavable ICAT® Reagent Kit Troubleshooting Guide (408k PDF)

Protein Sequencing

Procise® Cartridge Sensor Error Troubleshooting Tree (49k PDF)

Procise® PTH Chromatography Troubleshooting Tree (50k PDF)

Procise® PTH Computer Error Message Troubleshooting Tree (39k PDF)

Procise® Flask Sensor Errors Troubleshooting Tree (54k PDF)

Real-Time PCR

Getting Started with Real-Time PCR


• New to Real-Time PCR? Start here!
• Essentials of Real-Time PCR (153k PDF)
• Real-Time PCR: Understanding CT (2.03MB PDF)
• Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines (329k PDF)



Training


• Real-Time PCR Training
• Training Calendar
• Self-Paced Training
• Video Demonstrations and Pre-recorded Training
• Product Webinars
• Loading and Sealing Taqman Arrays
• Register for a Taqman® OpenArray®
  Genotyping System Training CD



Applications and Workflow


• Chemistry Guide: Applied Biosystems Real-Time PCR Systems (1.74 MB PDF)
• Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative
• Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR (287k PDF)



Genomic Assays, Primer and Probe Design


• Searching and Ordering Guide for TaqMan® Gene Expression Assays (2.24 MB PDF)
• Bioinformatic Evaluation of a Sequence for Custom TaqMan® Gene Expression Assays (1.5 MB PDF)
• Bioinformatic Evaluation of a Sequence for Custom TaqMan® SNP Genotyping Assays (4.31 MB PDF)
• TaqMan® Drug Metabolism Genotyping Assays: Reference Guide (2.3 MB PDF)
• Reconstituting and Diluting Primers and TaqMan® Probes (101k PDF)
• Load, Centrifuge and Seal TaqMan® Arrays (Flash Movie)
• User Bulletin: Running TaqMan® Custom Arrays on 7900HT Real-Time PCR Systems: Applied Biosystems TaqMan® Custom Array: Rev A (PDF) 873KB
• Primer Express® Software



Instrument and Software Guides




• StepOne™ and StepOnePlus™ Systems
• 7300, 7500, and 7500 Fast Systems (SDS v1.4)


7500 and 7500 Fast Systems (7500 Software v2.0, 2.1)


• User Bulletin: Memory Upgrade Requirements for 7500 Software v2.0 and v2.0.1
• Standard Curve Experiments
• Relative Standard Curve and Comparative CT Experiments
• Genotyping Experiments
• Presence/Absence Experiments
• Computer Setup Guide



7700 Sequence Detection System


• Setting-up Singleplex (single probe) Real Time 5' Nuclease Reactions on the ABI PRISM® 7700 Sequence Detection System
• Setting-up a Multiplex (two probe) Real Time 5' Nuclease Reaction on the ABI PRISM® 7700 Sequence Detection System (PDF, 410 KB)
• Setting-up Real-Time PCR Assays using SYBR® Green Dye on the ABI PRISM® 7700 Sequence Detection System
• Generating ABI PRISM® 7700 SDS Standard Curve Plots in a Spreadsheet Program (PDF, 317 KB)
• Data Analysis on the ABI PRISM® 7700 SDS: Setting Baselines and Thresholds (PDF, 312 KB)
• Generating ABI PRISM® 7700 SDS Amplification Plots in a Spreadsheet Program



7900HT Fast System


• Relative Quantitation Using Comparative CT (5.06 MB PDF)
• Allelic Discrimination (8.40 MB PDF)
• Absolute Quantitation Using Standard Curve (4.65 MB PDF)
• Plus/Minus Assay (7.89 MB PDF)
• Presence/Absence Experiments
• Computer Setup Guide